|
Prof.
Wolfgang Garten and colleagues are studying the
structure, biosynthesis, and function of the glycoprotein spikes of
enveloped viruses. In this context, the group of Prof. Klenk and Prof.
Garten has studied a variety of viruses, including influenza viruses,
various paramyxoviruses, coronaviruses, Ebola-Marburg viruses, Borna virus,
HIV, Lassa virus, and cytomegalovirus. The main research topics are the co-
and posttranslational modifications of viral spike proteins, such as
glycosylation, proteolytic processing, and acylation, in relation to their
biological functions and characteristics, mainly receptor binding, fusion,
and antigenicity. The research of Prof. Garten contributed to the discovery
of (i) proteolytic processing the viral glycoproteins as a prerequisite for
fusion, (ii) viral glycoprotein-processing proteases of host cells, (iii)
protease inhibition to achieve inhibition of fusion and, thereby, of virus
propagation. The group has experience in virology, molecular biology, and
cell biology over many years. They published more than 380 articles in
peer-reviewed journals. All techniques necessary for the project have been
established in the Marburg
laboratory, where researchers have extensive expertise with high-risk
viruses and access to a Bio-safety Level 4 laboratory. Dr. Markus Eickmann,
who is also involved in these studies, supervises the Molecular Virus
Diagnostics at the Institute.
Selected
publications of Partner 2:
- Song, H.C., Seo, M.Y., Stadler,
K., Yoo, B.J., Choo, Q.L., Coates, S.R., Uematsu, Y., Harada, T.,
Greer, C.E., Polo, J.M., Pileri, P., Eickmann, M., Rappuoli, R.,
Abrignani, S., Houghton, M., Han, J.H. 2004. Synthesis and
characterization of a native, oligomeric form of recombinant severe
acute respiratory syndrome coronavirus spike glycoprotein. J. Virol.
78: 10328-10335.
- Eickmann, M., Becker, S.,
Klenk, H.-D., Doerr, H.W., Stadler, K., Censini, S., Guidotti, S.,
Masignani, V., Scarselli, M., Mora, M., Donati, C., Han, J.H., Song,
H.C., Abrignani, S., Covacci, A., Rappuoli, R. 2003. Phylogeny of the
SARS coronavirus. Science 302: 1504-1505.
- Berting
A., Fischer C., Schaefer S., Garten W., Klenk H.D., and Gerlich W.H.
2000. Hemifusion
activity of a chimeric influenza virus hemagglutinin with a putative
fusion peptide from hepatitis B virus. Virus Res. 68:35-49.
- Boycott R., Klenk H.D.,
and Ohuchi M. 1994. Cell tropism of influenza virus mediated by
hemagglutinin activation at the stage of virus entry. Virology
203:313-319.
- Drosten C., Gunther S.,
Preiser W., van der Werf S., Brodt H.R., Becker S., Rabenau H.,
Panning M., Kolesnikova L., Fouchier R.A., Berger A., Burguiere A.M.,
Cinatl J., Eickmann M., Escriou N., Grywna K., Kramme S., Manuguerra
J.C., Muller S., Rickerts V., Sturmer M., Vieth S., Klenk H.D.,
Osterhaus A.D., Schmitz H., and Doerr H.W. 2003. Identification of a
novel coronavirus in patients with severe acute respiratory syndrome.
N. Engl. J. Med. 348:1967-1976.
- Fischer C., Schroth-Diez
B., Herrmann A., Garten W., and Klenk H.D. 1998. Acylation of the
influenza hemagglutinin modulates fusion activity. Virology
248:284-294.
- Klenk H.D., and Garten,
W. 1994. Activation cleavage of viral spike proteins. In:
Cellular Receptors for Animal Viruses. Monograph 28 (E.
Wimmer, ed.). Cold Spring Habor Laboratory Press, 241-280.
Prof.dr.
Wolfgang Garten
garten@staff.uni-marburg.de
Prof. Hans
Dieter Klenk
Klenk@staff.uni-marburg.de
Dr. Markus
Eickmann
eickmann@staff.uni-marburg.de
Markus Eickmann - Melanie Gössl - Jens Kursawe
Wolfgang Garten
Hans Dieter Klenk
|
