|
Prof.
Stuart G. Siddell has studied the biology and pathogenesis of human
and animal coronaviruses for over 20 years. His research has contributed to
the discovery of coronavirus discontinuous transcription, the development
of reverse genetic systems for human, avian and murine coronaviruses and
the elucidation of coronavirus protein structures. His expertise is to use
a combination of classical and reverse genetics to elucidate the
function(s) of the coronavirus replication-transcription complex proteins.
Professor Siddell uses a multidisciplinary approach and he has co-ordinated
successful research programmes over an extended period of time.
Dr.
Andrew D. Davidson has over ten years experience working in the field
of positive strand RNA virus reverse genetic systems. He developed and
routinely uses a dengue virus reverse genetic system to understand dengue
virus replication and pathogenesis. Dr Davidson and Professor Siddell work
together in the Department of Cellular and Molecular Medicine at the University of Bristol. Recently, Dr Davidson has
expanded his coronavirus research whilst maintaining his interest in dengue
virus. He has established the demanding techniques required for working
with RNA virus reverse genetic systems in the laboratory and is in charge
of a Bio-safety Level 3 laboratory, technology and facilities that is
essential to achieve the objectives of the Network.
Dr.
Chang Guo Hai has studied the SARS coronavirus at the Beijing
Institute of Microbiology and Epidemiology. He isolated and sequenced
several rare (minor genotype) Chinese strains of SARSCoV and was involved
in the development of an inactivated SARSCoV vaccine. He has experience in
the handling of live SARSCoV and is interested in the use of reverse
genetics to produce attenuated recombinant viruses.
Selected
publications of Partner 3:
- Sawicki S.G., Sawicki
D.L., Younker D., Meyer V., Thiel V., Stokes H., and Siddell S.G.
2005. Functional and genetic analysis of coronavirus
replicase-transcriptase proteins. PloS Pathogens, in press.
- Jones M, Davidson A,
Hibbert L, Gruenwald P, Schlaak J, Ball S, Foster GR,Jacobs M. 2005.
Dengue virus inhibits alpha interferon signalling by reducing STAT2
expression. J Virol. 79:5414-20.
- Davidson A.D., and
Siddell, S.G. 2003. Potential for antiviral treatment of severe acute
respiratory syndrome. Curr. Opinion Inf. Dis. 16: 565-571.
- Qin E, et al. 2003. A
genome sequence of novel SARS-CoV isolates: the genotype, GD-Ins29,
leads to a hypothesis of viral transmission in South
China. Genomics Proteomics Bioinformatics. 1:101-7.
- Chambers T.J, Liang Y.,
Droll D.A., Schlesinger J.J., Davidson A.D., Wright P.J., and Jiang X.
2003. Yellow fever virus/dengue-2 virus and yellow fever
virus/dengue-4 virus chimeras: biological characterisation,
immunogenicity, and protection against dengue encephalitis in the
mouse model. J. Virol. 77:3655-3668.
- Thiel V., Herold J.,
Schelle B., and Siddell S.G. 2001. Infectious RNA transcribed in vitro
from a cDNA copy of the Human coronavirus genome cloned in Vaccinia
virus. J. Gen. Virol. 82:1273-1281.
Prof. Stuart G. Siddell
stuart.siddell@bristol.ac.uk
Dr. Andrew D. Davidson
andrew.davidson@bristol.ac.uk
Dr Chang Guo Hai
chang.guohai@bristol.ac.uk
|
